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大鼠脂蛋白(Lp-a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK11055
  • Price:Negotiable
  • Clicks:2354

Product Detail

Product Information

Product NameRat Lp-a (Lipoprotein A) ELISA Kit
Alternative NamesLP; AK38; APOA

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.131 µg/mL
Detection Range0.312-20 µg/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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