| Product Name | Rat Lp-a (Lipoprotein A) ELISA Kit |
|---|---|
| Alternative Names | LP; AK38; APOA |
| Species | Rat |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.131 µg/mL |
| Detection Range | 0.312-20 µg/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve. |
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