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大鼠血管内皮钙黏蛋白(VE-Cadherin)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10792
  • Price:Negotiable
  • Clicks:2162

Product Detail

Product Information

Product NameRat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Alternative NamesCD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity1.21 ng/mL
Detection Range3.13-200 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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