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绵羊分泌型免疫球蛋白A(sIgA)酶联免疫吸附检测试剂盒

  • 货号:ELK0009
  • Price:USD $ 2,800.00
  • 点击:2143

Product Detail

产品信息

英文名称Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit
别名S-IgA

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Sheep
检测类型Sandwich
灵敏度0.049 µg/mL
检测范围0.16-10 µg/mL
样本类型serum, plasma, saliva and other biological fluids
实验时长3.5h

研究信息

研究领域Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep sIgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep sIgA in the samples is then determined by comparing the OD of the samples to the standard curve.

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