大鼠脂蛋白关联磷脂酶A2(LpPLA2)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10630MS
- Price：Negotiable
- Clicks：1865
Product Detail
Product Information
| Product Name | Rat LpPLA2(Phospholipase A2, Lipoprotein Associated) Microsample ELISA Kit |
|---|
| Alternative Names | PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2 |
|---|
Assay Parameters
| Species | Rat |
|---|
| Assay Type | Sandwich |
|---|
| Sensitivity | 5.3 pg/mL |
|---|
| Detection Range | 3.13-200 ng/mL |
|---|
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
|---|
| Assay Length | 3.5h |
|---|
Research Information
| Research Area | Enzyme & Kinase;Metabolic pathway;Infection immunity; |
|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LpPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LpPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LpPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
Standard Curve
| Standard Curve |  |
|---|
