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小鼠肌球蛋白重链2(MYH2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10364
  • Price:Negotiable
  • Clicks:2146

Product Detail

Product Information

Product NameMouse MYH2(Myosin Heavy Chain 2, Skeletal Muscle, Adult) ELISA Kit
Alternative NamesMYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.049 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Developmental science;Bone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MYH2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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