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人极低密度脂蛋白受体(VLDLR)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10061
  • Price:Negotiable
  • Clicks:2012

Product Detail

Product Information

Product NameHuman VLDLR(Very Low Density Lipoprotein Receptor) ELISA Kit
Alternative NamesVLDL-R

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.31 ng/mL
Detection Range1.25-80 ng/mL
Sample Typeserum, plasma,Tissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VLDLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VLDLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VLDLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VLDLR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP98155

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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