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一步法人白介素2受体a链(IL-2Ra/CD25)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK087ES
  • Price:Negotiable
  • Clicks:1656

Product Detail

Product Information

Product NameEasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Alternative NamesIL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity37.66 pg/mL
Detection Range93.75-6000 pg/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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