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小鼠信号素4C(SEMA4C)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0841
  • Price:Negotiable
  • Clicks:2494

Product Detail

Product Information

Product NameMouse SEMA4C(Semaphorin 4C) ELISA Kit
Alternative NamesSEMAF; SEMAI; SEMACL1; M-SEMA-F

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.057 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA4C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SEMA4C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SEMA4C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SEMA4C in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ64151

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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