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一步法人血管性血友病因子(vWF)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK074ES
  • Price:Negotiable
  • Clicks:2340

Product Detail

Product Information

Product NameEasyStep Human vWF(Von Willebrand Factor) ELISA Kit
Alternative NamesF8VWF; VWD; von Willebrand antigen 2

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity3.97 ng/mL
Detection Range15.63-1000 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaSignal transduction;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWF, and the Human vWF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human vWF. After TMB substrate solution is added, only those wells that contain Human vWF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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