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小鼠肌原纤蛋白1(FBN1)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0723MS
  • Price:Negotiable
  • Clicks:2552

Product Detail

Product Information

Product NameMouse FBN1(Fibrillin 1) Microsample ELISA Kit
Alternative NamesFBN; MASS; MFS1; OCTD; SGS;  Asprosin

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.062 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Developmental science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FBN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FBN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FBN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FBN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ61554

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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