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人6酮前列腺素F1α(6-K-PGF1α)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0571
  • Price:Negotiable
  • Clicks:2352

Product Detail

Product Information

Product NameHuman 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit
Alternative Names6-keto-PGF1 alpha; 6ketoPGF1α

Assay Parameters

SpeciesHuman
Assay TypeCompetitive Inhibition
Sensitivity5.4 pg/mL
Detection Range12.5-800 pg/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length2.5h

Research Information

Research AreaNeuro science;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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