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猫趋化因子C-X-C-基元受体4(CXCR4)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0535
  • Price:Negotiable
  • Clicks:2012

Product Detail

Product Information

Product NameCat CXCR4(Chemokine C-X-C-Motif Receptor 4) ELISA Kit
Alternative NamesCD184; Fusin; NPYR; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYRL; WHIM; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3

Assay Parameters

SpeciesCat
Assay TypeSandwich
Sensitivity37.8 pg/mL
Detection Range78.13-5000 pg/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cat CXCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cat CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cat CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cat CXCR4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP56498

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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