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一步法人游离三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK048ES
  • Price:Negotiable
  • Clicks:2312

Product Detail

Product Information

Product NameEasyStep Human fT3(Free Triiodothyronine) ELISA Kit
Alternative Names3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Assay Parameters

SpeciesHuman
Assay TypeCompetitive Inhibition
Sensitivity0.31 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaEndocrinology;Autoimmunity;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT3 antigen, and the Human fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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