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人基质细胞衍生因子2(SDF2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0465
  • Price:Negotiable
  • Clicks:2278

Product Detail

Product Information

Product NameHuman SDF2(Stromal Cell Derived Factor 2) ELISA Kit
Alternative NamesSDF-2

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity13.4 pg/mL
Detection Range31.25-2000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SDF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SDF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SDF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ99470

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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