小鼠角蛋白14(KRT14)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0247
- Price：Negotiable
- Clicks：2242
Product Detail
Product Information
| Product Name | Mouse KRT14(Keratin 14) ELISA Kit |
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| Alternative Names | CK14; EBS3; EBS4; K14; Cytokeratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin, type I cytoskeletal 14 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.061 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KRT14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KRT14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KRT14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KRT14 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q61781 |
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Standard Curve
| Standard Curve |  |
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