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大鼠内皮素转化酶1(ECE1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0229
  • Price:USD $ 2,800.00
  • Clicks:1599

Product Detail

Product Information

Product NameRat ECE1(Endothelin Converting Enzyme 1) ELISA Kit
Alternative NamesECE1; ECE-1

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity22.1 pg/mL
Detection Range78.13-5000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ECE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ECE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ECE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ECE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP42893

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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