8异前列腺素F2α(8-iso-PGF2a)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0151
- Price：Negotiable
- Clicks：2557
Product Detail
Product Information
| Product Name | 8-iso-PGF2α(8-isoprostane) ELISA Kit |
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| Alternative Names | 8-isoprostane; 8-iso-PGF2α |
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Assay Parameters
| Species | General |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 4.47 pg/mL |
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| Detection Range | 15.63-1000 pg/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 2.5h |
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Research Information
| Research Area | Endocrinology;Pulmonology;Hepatology;Hormone metabolism; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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