人精子粘附分子1(SPAM1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0145
- Price：Negotiable
- Clicks：2551
Product Detail
Product Information
| Product Name | Human SPAM1(Sperm Adhesion Molecule 1) ELISA Kit |
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| Alternative Names | HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 4.22 pg/mL |
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| Detection Range | 15.63-1000 pg/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Reproductive science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPAM1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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