大鼠绒毛膜促性腺激素(hCG)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0429
- Price：Negotiable
- Clicks：2868
Product Detail
Product Information
| Product Name | Rat CG(Chorionic Gonadotropin) ELISA Kit |
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| Alternative Names | CG; Chorionic Gonadotrophin |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.188 mIU/mL |
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| Detection Range | 1.25-80 mIU/mL |
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| Sample Type | serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CG in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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