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一步法人血管细胞粘附分子1(VCAM1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK043ES
  • Price:Negotiable
  • Clicks:3016

Product Detail

Product Information

Product NameEasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
Alternative NamesCD106; INCAM-100; L1CAM

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.87 ng/mL
Detection Range1.25-80 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaCD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP19320

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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