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鸡谷胱甘肽过氧化物酶3(GPX3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0130
  • Price:Negotiable
  • Clicks:2465

Product Detail

Product Information

Product NameChicken GPX3(Glutathione Peroxidase 3) ELISA Kit
Alternative NamesGSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase

Assay Parameters

SpeciesChicken
Assay TypeSandwich
Sensitivity21.2 pg/mL
Detection Range78.13-5000 pg/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GPX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken GPX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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