Anti-G protein/Gαβγ Recombinant Nanobody (Nb35)
- Cat. No.：RB9THX948013
- Price：Negotiable
- Clicks：477
Product Detail
Product Parameters
| Form | Liquid |
|---|---|
| Host Species | Alpaca |
| Clonality | Monoclonal |
| Isotype | VHH-His |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin Level | Please contact with the lab for this information. |
| Applications | Crystallography, Cryo-EM |
| Species Reactivity | Homo sapiens (Human) |
| Target | Adenylate cyclase-stimulating G alpha protein, EC:3.6.5.-, GNAS, GNAS1, GSP, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
| Storage Buffer | 0.01M PBS pH 7.4 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Accession Number | P63092 |
| Background | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Gαβγ) is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. 1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161 2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384 3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135 4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488 5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665 6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251 |
| Note | For research use only |
Related Products: