马核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒
- Cat. No.：ELK9972
- Price：Negotiable
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Product Detail
Product Information
| Product Name | Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit |
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| Alternative Names | NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B |
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Assay Parameters
| Species | Horse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.059 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | F6TME6 |
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Standard Curve
| Standard Curve |  |
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