人SBDP150蛋白(SBDP 150)酶联免疫吸附检测试剂盒
- Cat. No.：ELK9885
- Price：Negotiable
- Clicks：2251
Product Detail
Product Information
| Product Name | Human SBDP 150(Alpha II SPECTRIN BREAKDOWN PRODUCT 150) ELISA Kit |
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| Alternative Names | SBDP150; SBDP-150; spectrin alpha, non-erythrocytic 1 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.124 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SBDP 150. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SBDP 150. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SBDP 150, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SBDP 150 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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