可的松(Cortisone)酶联免疫吸附检测试剂盒
- Cat. No.:ELK11222
- Price:Negotiable
- Clicks:1625
Product Detail
Product Information
| Product Name | Cor(Cortisone) ELISA Kit |
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| Alternative Names | 17OHCS; 17-OHCS; 17-Hydroxy-11-Dehydrocorticosterone; 17-Hydroxycorticosteroid |
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Assay Parameters
| Species | General |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 0.79 ng/mL |
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| Detection Range | 3.13-200 ng/mL |
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| Sample Type | serum plasma and other biological fluids |
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| Assay Length | 2h |
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Research Information
| Research Area | Metabolism; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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For Research Use Only. Not for Diagnostic Procedures.
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