猪可溶性末端补体复合物sC5b-9(sC5b-9)酶联免疫吸附检测试剂盒
- Cat. No.：ELK11184
- Price：Negotiable
- Clicks：2055
Product Detail
Product Information
| Product Name | Pig sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit |
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| Alternative Names | MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9 |
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Assay Parameters
| Species | Pig |
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| Assay Type | Sandwich |
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| Sensitivity | 12.91 ng/mL |
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| Detection Range | 31.25-2000 ng/mL |
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| Sample Type | Plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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