豚鼠血红蛋白(HB)酶联免疫吸附检测试剂盒
- Cat. No.：ELK11073
- Price：Negotiable
- Clicks：1709
Product Detail
Product Information
| Product Name | Guinea pig HB(Hemoglobin) ELISA Kit |
|---|---|
| Alternative Names | Hgb; Haemoglobin; Heterotetramer(αβ)2 |
Assay Parameters
| Species | Guinea pig |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.69 µg/mL |
| Detection Range | 1.56-100 µg/mL |
| Sample Type | Serum, plasma, erythrocyte lysates. |
| Assay Length | 3.5h |
Research Information
| Research Area | Metabolic pathway;Infection immunity;Hematology;Neuro science; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig HB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig HB in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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