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人B-淋巴细胞趋化因子1(BLC1)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK1106MS
  • Price:Negotiable
  • Clicks:2000

Product Detail

Product Information

Product NameHuman BLC1(B-Lymphocyte Chemoattractant 1) Microsample ELISA Kit
Alternative NamesCXCL13; SCYB13; BLC; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine(C-X-C-Motif)ligand 13; Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity6.3 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;Immune molecule;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BLC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BLC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BLC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BLC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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