大鼠骨涎蛋白(BSP)酶联免疫吸附检测试剂盒

Product Name	Rat BSP(Bone Sialoprotein) ELISA Kit
Alternative Names	IBSP; Bsp; BspII; SpII; Integrin-Binding Bone Sialoprotein; Cell-binding sialoprotein; Bone sialoprotein 2

Species	Rat
Assay Type	Sandwich
Sensitivity	0.22 ng/mL
Detection Range	0.78-50 ng/mL
Sample Type	Serum, plasma, tissue homogenates and other biological fluids
Assay Length	3.5h

Research Area	Metabolic pathway;Developmental science;Bone metabolism;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BSP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P13839

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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