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人可溶性转铁蛋白受体(sTFR)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10988
  • Price:Negotiable
  • Clicks:1640

Product Detail

Product Information

Product NameHuman sTFR(Soluble Transferrin Receptor) ELISA Kit
Alternative NamesCD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.57 ng/mL
Detection Range4.69-300 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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