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人胸腺基质淋巴细胞生成素(TSLP)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK1083MS
  • Price:Negotiable
  • Clicks:1910

Product Detail

Product Information

Product NameHuman TSLP(Thymic Stromal Lymphopoietin) Microsample ELISA Kit
Alternative NamesTslp

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity6.9 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaImmune molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSLP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSLP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSLP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSLP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ969D9

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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