小鼠羧肽酶B1(CPB1)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10808MS
- Price：Negotiable
- Clicks：1856
Product Detail
Product Information
| Product Name | Mouse CPB1(Carboxypeptidase B1, Tissue) Microsample ELISA Kit |
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| Alternative Names | CPB; PCPB; PASP; Pancreas-specific protein |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.27 ng/mL |
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| Detection Range | 0.79-50 ng/mL |
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| Sample Type | Serum and plasma. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CPB1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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