大鼠骨骼肌慢肌肌钙蛋白I(TNNI1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10738
- Price：Negotiable
- Clicks：2112
Product Detail
Product Information
| Product Name | Rat TNNI1(Troponin I Type 1, Slow Skeletal) ELISA Kit |
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| Alternative Names | TNN1; Troponin I, slow-twitch isoform |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 18.3 pg/mL |
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| Detection Range | 78.13-5000 pg/mL |
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| Sample Type | Serum, plasma and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNNI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNNI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNNI1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P13413 |
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Standard Curve
| Standard Curve |  |
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