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大鼠胃动蛋白1(GKN1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10737
  • Price:Negotiable
  • Clicks:2383

Product Detail

Product Information

Product NameRat GKN1(Gastrokine 1) ELISA Kit
Alternative NamesCA11; AMP18; BRICD1; FOV; Foveolin; 18 kDa antrum mucosa protein

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity4.86 ng/mL
Detection Range15.63-1000 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaTumor immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GKN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GKN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GKN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GKN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ6SJV7

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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