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大鼠多配体蛋白聚糖4(SDC4)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10714
  • Price:Negotiable
  • Clicks:1760

Product Detail

Product Information

Product NameRat SDC4(Syndecan 4) ELISA Kit
Alternative NamesSYND4; Amphiglycan; Ryudocan; Ryudocan core protein

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.14 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;Infection immunity;Bone metabolism;Rheumatology;Immunodeficiency;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SDC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SDC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SDC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SDC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP34901

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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