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牛血小板反应蛋白1(TSP-1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10705
  • Price:Negotiable
  • Clicks:2400

Product Detail

Product Information

Product NameCattle TSP-1(Thrombospondin-1) ELISA Kit
Alternative NamesTSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

Assay Parameters

SpeciesCattle
Assay TypeSandwich
Sensitivity0.43 ng/mL
Detection Range1.56-100 ng/mL
Sample TypeSerum and plasma
Assay Length3.5h

Research Information

Research AreaInfection immunity;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ28178

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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