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牛胰岛素样生长因子结合蛋白6(IGFBP6)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10702
  • Price:Negotiable
  • Clicks:1575

Product Detail

Product Information

Product NameCattle IGFBP6(Insulin Like Growth Factor Binding Protein 6) ELISA Kit
Alternative NamesIBP6; IGF-binding protein 6

Assay Parameters

SpeciesCattle
Assay TypeSandwich
Sensitivity0.063 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaTumor immunity;Endocrinology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IGFBP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IGFBP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP6 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ05718

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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