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牛胰岛素样生长因子结合蛋白4(IGFBP4)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10693
  • Price:Negotiable
  • Clicks:2100

Product Detail

Product Information

Product NameCattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
Alternative NamesBP4; HT29-IGFBP; IBP4

Assay Parameters

SpeciesCattle
Assay TypeCompetitive Inhibition
Sensitivity0.56 ng/mL
Detection Range1.57-100 ng/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length2h

Research Information

Research AreaApoptosis;Tumor immunity;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ05716

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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