| Product Name | Rat Dyn(Big Dynorphin) ELISA Kit |
|---|---|
| Alternative Names | B-DYN; Biocytin 13 Dynorphin A 1-13 Amide |
| Species | Rat |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 5.8 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Signal transduction;Neuro science; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Dyn. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Dyn. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Dyn in the samples is then determined by comparing the OD of the samples to the standard curve. |
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