牛乳铁传递蛋白(LTF)酶联免疫吸附检测试剂盒

Product Name	Cattle LTF(Lactoferrin) ELISA Kit
Alternative Names	HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

Species	Cattle
Assay Type	Sandwich
Sensitivity	17.6 ng/mL
Detection Range	78.13-5000 ng/mL
Sample Type	serum, plasma, tissue homogenates, cell lysates, saliva, milk,cell culture supernates and other biological fluids
Assay Length	3.5h

Research Area	Tumor immunity;Infection immunity;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P24627

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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