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小鼠激肽释放酶1(KLK1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10619
  • Price:Negotiable
  • Clicks:2519

Product Detail

Product Information

Product NameMouse KLK1(Kallikrein 1) ELISA Kit
Alternative NamesKLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Assay Parameters

SpeciesMouse
Assay TypeCompetitive Inhibition
Sensitivity0.14 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length2h

Research Information

Research AreaEnzyme & Kinase;Tumor immunity;Hepatology;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP15947

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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