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人补体成分1q子成分C(C1qC)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10556
  • Price:Negotiable
  • Clicks:1514

Product Detail

Product Information

Product NameHuman C1qC(Complement Component 1, Q Subcomponent C) ELISA Kit
Alternative NamesC1QG; Complement Component 1 q subcomponent,Gamma Polypeptide

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.29 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaImmune molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1qC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1qC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1qC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1qC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP02747

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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