人骨膜蛋白(POSTN)微量上样酶联免疫吸附检测试剂盒
- Cat. No.:ELK10527MS
- Price:Negotiable
- Clicks:1500
Product Detail
Product Information
| Product Name | Human POSTN(Periostin) Microsample ELISA Kit |
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| Alternative Names | OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 2.07 ng/mL |
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| Detection Range | 7.82-500 ng/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human POSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human POSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POSTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q15063 |
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Standard Curve
| Standard Curve |  |
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For Research Use Only. Not for Diagnostic Procedures.
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