小鼠B细胞活化因子(BAFF/CD257)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10525
- Price：Negotiable
- Clicks：2470
Product Detail
Product Information
| Product Name | Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit |
|---|
| Alternative Names | CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF |
|---|
Assay Parameters
| Species | Mouse |
|---|
| Assay Type | Sandwich |
|---|
| Sensitivity | 0.067 ng/mL |
|---|
| Detection Range | 0.16-10 ng/mL |
|---|
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
|---|
| Assay Length | 3.5h |
|---|
Research Information
| Research Area | CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
| UniProt ID | Q9WU72 |
|---|
Standard Curve
| Standard Curve |  |
|---|
