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小鼠半乳糖凝集素3(GAL3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10486
  • Price:Negotiable
  • Clicks:2234

Product Detail

Product Information

Product NameMouse GAL3(Galectin 3) ELISA Kit
Alternative NamesLGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.062 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP16110

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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