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人血栓调节蛋白(TM)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10448
  • Price:Negotiable
  • Clicks:2370

Product Detail

Product Information

Product NameHuman TM(Thrombomodulin) ELISA Kit
Alternative NamesCD141; THBD; BDCA3; THRM; Fetomodulin

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.052 ng/mL
Detection Range0.16-10 ng/mL
Sample TypePlasma
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Hematology;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP07204

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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