小鼠基质细胞衍生因子1(SDF1)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10399MS
- Price：Negotiable
- Clicks：2261
Product Detail
Product Information
| Product Name | Mouse SDF1(Stromal Cell Derived Factor 1) Microsample ELISA Kit |
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| Alternative Names | CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.054 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Cytokine;Tumor immunity;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDF1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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