人Ⅰ类主要组织相容性复合体E(MHCE)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10370MS
- Price：Negotiable
- Clicks：2228
Product Detail
Product Information
| Product Name | Human MHCE(Major Histocompatibility Complex Class I E) Microsample ELISA Kit |
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| Alternative Names | HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.12 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCE in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P13747 |
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Standard Curve
| Standard Curve |  |
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