人高尔基蛋白73(GP73)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10367MS
- Price：Negotiable
- Clicks：2288
Product Detail
Product Information
| Product Name | Human GP73(Golgi Protein 73) Microsample ELISA Kit |
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| Alternative Names | GOLM1; GOLPH2; C9orf155; Golgi Membrane Protein 1; Golgi Phosphoprotein 2 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 5.06 ng/ml |
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| Detection Range | 6.25-400 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity;Infection immunity;Reproductive science;Hepatology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GP73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GP73, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GP73 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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